Light augments FOS protein induction in brain of short-term enucleated hamsters

Abstract

FOS protein is synthesized in neuronal nuclei in response to a variety of environmental stimuli and has been used as a marker of stimulus-specific brain function. The present studies were initiated to examine the effects of ultraviolet light on the induction of FOS protein immunoreactivity (FOS-IR) in several brain regions of adult male hamsters. Experiment 1 confirmed previous observations of FOS-IR induced in visual cortex in response to ultraviolet light. However, protein was also induced by ultraviolet or white light in a variety of other areas and induction occurred in both sighted and enucleated animals. Therefore, experiments were conducted to evaluate the effects of a 514 nm light on FOS-IR induction in blind or sighted animals. Experiments 2 and 3 were performed during the early subjective night and mid-subjective day, respectively, using animals about 4 days after bilateral enucleation or sham surgery. In Experiment 2, light and enucleation independently and interactively resulted in increased FOS-IR neuronal nuclei counts. In Experiment 3, there was a main effect of enucleation and an interaction between enucleation and light condition, but no main effect of light. In Experiment 4, conducted during the early subjective night using animals enucleated 60 days earlier, there were neither effects of light or enucleation. The results support the view that, under certain conditions related to subjective time of day and time since enucleation, light can act through unknown extraocular mechanisms to modify brain activity. Further, short term enucleation itself induces widespread alteration in brain function as indicated by increased FOS-IR expression. The results specifically do not support a role for extraretinal photoreception with respect to direct circadian rhythm regulation.