Abstract
The infection ofFrankliniella occidentalisby two isolates of the entomopathogenic fungusMetarhizium anisopliaewas studied using fluorescence, scanning, transmission, and confocal scanning laser microscopy techniques. Conidia ofM. anisopliaeadhered mostly to the wings of adultF. occidentalisbut the number declined from 73 to 40% within 72 h postinoculation at 23°C, presumably due to preening. Conidia germinated and produced appressoria on adult, larval, and pupal stages within 12 h post inoculation. However, penetration pegs were not observed until 30 h postinoculation. Light microscopy and transmission electron microscopy provided further information on fungal development inside the insect. The fungus colonized the insect hemocoel from day 3 and sporulated approximately 6 days postinoculation.
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