Light and electron microscopic studies on short-term preserved corneae (author's transl)

Abstract

Rabbit corneae were stored at a temperature of +4 degrees C in the following media: 1. 5% Dextran T40, 2. McCoy's Medium 5a (modified), 3. Dextran T 40+McCoy's 5a Medium + gentamicin sulphate (250 mug/ml). The endothelia of the corneae were examined by light and electron microscopy after preservation for a varied number of days. The structure of the endothelia, stored for 1, 6, 12, 18 days, was compared to fresh corneal endothelial cells. The results indicate that corneae preserved in Dextran 5% T40 + McCoy's 5a Medium + gentamicin sulphate might be equivalent to fresh corneal tissue in keratoplasties.