Ligand-exchange chromatography of racemates : V. Separation of optical isomers of amino acids on a polystyrene resin containing l-hydroxyproline

Abstract

An asymmetric resin containing copper(II) ions with l-hydroxyproline ligands fixed in a macronet isoporous polystyrene matrix has been used for the separation of optical isomers of amino acids. The resin selectivity and the column efficiency were determined and their dependence on the chromatographic conditions (the degree of saturation of the resin with copper(II) ions, ammonia concentration, column parameters) discussed.