Abstract
Storage of hemoglobin-based-oxygen-carriers (HBOC) is crucial for its use as an oxygen delivery system in a wide range of climatic environments. Ideally storage should be under local ambient conditions and not dependent upon refrigeration. Serious concern must be given to alterations of the physiochemical and functional properties of an HBOC, including oxidation of the heme moiety, alteration of the structural stability, and changes in the O2 carrying capacity. OxyVita Hb, a zero-linked polymeric bovine hemoglobin, is a new generation HBOC that is presently stable for up to five years frozen at −80°C. Improved storage can be achieved by conversion of the oxyHb to a CO form. Stability at ambient temperature up to seven months has been achieved to date. Increased structural stability is observed, methemoglobin levels are significantly reduced, and no change in molecular size is observed. Removal of the CO ligand occurs at a faster rate from the polymeric form of OxyVita Hb than the cross-linked stroma free tetrameric COHb, whole bovine CO blood and bovine CO rbc. Faster kinetic exchange (CO→O2) by OxyVita Hb may be due to alteration of the secondary and tertiary structure of the incorporated tetramers into this large polymeric HBOC. Results from spectroscopic studies will be presented.
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