Abstract
Traditional laboratory biology is a small-scale operation; tissue culture dishes are seldom much larger than the experimenter's hand, volume measurements are in milliliters, and a protein purification that yields a few micrograms is a success. With burgeoning interest in translational research, structural biology, and regenerative medicine, many scientists are starting to think bigger. Whether trying to purify grams of protein for crystallography or testing the feasibility of turning a novel gene product into a new drug, these researchers soon find themselves pondering the complications of larger-scale cell culture.
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