Abstract
Peroxisomal shape, distribution, motility, and interactions with cytoskeletal elements were examined during interphase in living leek (Allium porrum L.) epidermal cells transiently transformed with a construct encoding the green fluorescent protein bearing a carboxy-terminal type 1 peroxisomal targeting signal. Confocal laser scanning microscopy and time-course analysis revealed that labeled peroxisomes were either spherical or rod-shaped and possessed several types of motility including random oscillations, slow and fast directional and bidirectional movements, and stop-and-go movements. Co-localization studies indicated that most peroxisomes were in close association with actin filaments, while treatment of cells with the actin-disrupting drug cytochalasin D blocked all types of peroxisomal movements. In contrast, the overall spatial organization of peroxisomes and the microtubule cytoskeleton were different, and the microtubule-destabilizing agent oryzalin had no obvious effect on peroxisomal motility. These data indicate that the peroxisome in plant cells is a highly dynamic compartment that is dependent upon the actin cytoskeleton, not microtubules, for its subcellular distribution and movements.Key words: actin filaments, cytoskeleton, green fluorescent protein, leek, microtubules, peroxisomes.
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