Life in acid: interactive effects of pH and natural organic acids on growth, development and locomotor performance of larval striped marsh frogs (Limnodynastes peronii)

Abstract

The low pH of naturally acidic aquatic environments is the result of soft-water with low buffering capacity and high concentrations of natural organic acids. Our current understanding of the influence of pH on aquatic organisms is largely limited to laboratory studies conducted under controlled conditions with little incorporation of these organic acids. Recent studies suggest natural organic acids may influence the physiology of aquatic species independent of low pH. We examined the effects of pH and varying concentrations of natural wallum water, which is high in organic acids on the hatching success, growth and locomotor performance of larval striped marsh frogs (Limnodynastes peronii). Based on previous studies, we predicted that the detrimental effects of low pH would be further exacerbated by higher concentrations of naturally occurring organic acids (high concentrations of wallum water). In artificial soft-water, embryos experienced both reduced growth and reduced survival when exposed to low pH. However, greater concentrations of natural organic acids did not exacerbate these effects of low pH on growth and development. Instead, we found some evidence that the natural organic acids within wallum water improved growth and swimming performance across all pH treatments. Using path analyses to investigate the effects of pH and natural organic acid concentration on burst swimming performance, we found performance was directly affected by both body length and organic acid concentration. Our data further highlight our limited understanding of the importance of natural organic acids for aquatic organisms and the need to incorporate greater ecological relevance into these studies.