Lidokain ve Bupivakain'in Tek Başına ve Fentanil ile Kombinasyonlarının Antibakteriyel Etkilerinin İn Vitro İncelenmesi

Abstract

Objective: It was aimed to investigate the in vitro antibacterial activities of the combined use of local anesthetic agents like lidocaine and bupivacaine, the antibacterial effects of which have been demonstrated, with fentanyl. Material and Methods: The in vitro antimicrobial activities of lidocaine, bupivacaine alone and in combination with fentanyl at different concentrations were investigated using microdilution technique. Microorganisms used in the test were Escherichia coli ATCC 25922, Yersinia pseudotuberculosis ATCC 911, Pseudomonas aeruginosa ATCC 10145, Listeria monocytogenes ATCC 43251, Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, Bacillus cereus 702 Roma, Mycobacterium smegmatis ATCC607, Candida albicans ATCC 60193, and Saccharomyces cerevisiae RSKK 251. Antibacterial assays were performed in Mueller-Hinton broth at pH 7.3 and antifungal analyses were performed in buffered Yeast Nitrogen Base at pH 7.0. Results: While lidocaine, bupivacaine, and fentanyl demonstrated antibacterial activity when they were used alone, no antibacterial effect was observed when they were used in combination. Conclusion: The antibacterial efficacy of both lidocaine and bupivacaine is evident when both local anesthetic agents are used alone. However, the antibacterial efficacy is reduced when both agents are combined with fentanyl, which shows that the risk of infection may be more likely.