Abstract
Licochalcone A (LicA), a flavonoid isolated from the famous Chinese medicinal herb Glycyrrhiza uralensis Fisch, has wide spectrum of pharmacological activities. In this study, the anti-cancer effects and potential mechanisms of LicA in non-small cell lung cancer (NSCLC) cells were studied. LicA decreased cell viability and induced apoptosis in a dose-dependent manner in NSCLC cells. LicA inhibited lung cancer cells growth by blocking cell cycle progression at the G2/M transition and inducing apoptosis. LicA treatment decreased the expression of MDM2, Cyclin B1, Cdc2 and Cdc25C in H460 and A549 cancer cell lines. In addition, LicA induced caspase-3 activation and poly-ADP-ribose polymerase (PARP) cleavage, which displayed features of apoptotic signals. Furthermore, LicA increased the expression of endoplasmic reticulum (ER) stress related proteins, such as p-EIF2α and ATF4. These data provide evidence that LicA has the potential to be used in the treatment of lung cancer.
Highlights
Non-small cell lung cancer (NSCLC) is a leading cause of cancer-related deaths worldwide [1]
We found that piperlongumine, a natural alkaloid isolated from the fruit of long pepper, selectively kills gastric cancer cells while sparing their normal counterparts [13]
To determine whether the growth inhibition of lung cancer cells by Licochalcone A (LicA) was caused by cell cycle arresTto, tdweotelrumnignecawncheerthceelrl tlhineesgrwoewrtehtrienahtiebditwiointhovf alurinoguscaconncecerncterlalstiobnysLoifcALicwAafsocra1u6sehd, tbhyencethlle ccyeclllecayrcrleeswt, atswdoeltuernmgicnaendcbery cfelollwlincyetsowmeerteryt.rTeahteedrewsuiltths vinarFioiguusreco2nAc–e2nCtrasthioonwsedofthLaictALifcoAr 1d6oshe,thdeenpetnhdeecnetlllycyincdleuwceads Gde2t/eMrmarinreesdt binytflhoewtwcoytloumngetcrayn. cTehrecreellssu. lts in Figure 2A–C showed that LicA dose-dependently induced G2/M arrest in the two lung cancer cells
Summary
Non-small cell lung cancer (NSCLC) is a leading cause of cancer-related deaths worldwide [1]. We clarified that two curcumin analogues selectively kill gastric cancer cells via activating ER stress pathway [14,15]. LicA E2f.feRcetsivueltlsy Suppressed the Proliferation of Human Lung Cancer Cells. LicA Induced G2/M Cell Cycle Arrest in Human Lung Cancer Cells. To determine whether the growth inhibition of lung cancer cells by LicA was caused by cell cycle arresTto, tdweotelrumnignecawncheerthceelrl tlhineesgrwoewrtehtrienahtiebditwiointhovf alurinoguscaconncecerncterlalstiobnysLoifcALicwAafsocra1u6sehd, tbhyencethlle ccyeclllecayrcrleeswt, atswdoeltuernmgicnaendcbery cfelollwlincyetsowmeerteryt.rTeahteedrewsuiltths vinarFioiguusreco2nAc–e2nCtrasthioonwsedofthLaictALifcoAr 1d6oshe,thdeenpetnhdeecnetlllycyincdleuwceads Gde2t/eMrmarinreesdt binytflhoewtwcoytloumngetcrayn. LLiiccAA iinndduucceess aappooppttoossiiss iinn hhuummaann lluunngg ccaanncceerr cceellllss.. All images shown here are representative of three independent experiments with similar results
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