Abstract
Brazil is known for its great potential for production of renewable resources such as agro-industrial residues. These residues can be used as alternative sources of new products. Meanwhile, solid-state fermentation, with its advantages of energy conservation and pollution reduction, has been identified as a process of great potential for the production of bioactive compounds, especially enzymes. In the present work, a 23 factorial design was used to evaluate the effects of pH, temperature and moisture on the production of phytase and xylanase by Lichtheimia blakesleeana URM 5604 through the fermentation of citrus pulp. Statistical analyses of the results showed that the only the pH influenced the production of these enzymes, with the best phytase production (264.68 U/g) ocurring at pH 6.0, 34 °C, initial moisture 50%, after 48 hours of culture. The best conditions for xylanase production (397.82 U/g) were fermentation for 120 hours at pH 4.0, 26 °C and initial moisture of 70%. The best parameters for the simultaneous production of phytase (226.92 U/g) and xylanase (215.59 U/g) were determined to be initial moisture of 50%, pH 6.0, 26 °C, and 48 hours of fermentation.
Highlights
Large numbers of microorganisms, including bacteria, yeasts and fungi, have been used in solid state fermentation (SSF) systems [1]
The Lichtheimia blakesleeana URM5604 used in this work has not been previously described as a producer of phytases and/or xylanases
We evaluated the enzyme production using solid-state fermentation at different pH, temperature and initial moisture values with citrus pulp as a substrate
Summary
Large numbers of microorganisms, including bacteria, yeasts and fungi, have been used in solid state fermentation (SSF) systems [1]. The use filamentous fungi for the production of commercially important products through in SSF has attracted much research interest during recent years [2,3]. Various fungi such as Thermomucor spp., Mucor spp., Aspergillus spp. and Rhizopus spp. have been used to produce various enzymes (cellulases, xylanases, ligninases, and pectinases), proteins and organic acids that can increase the digestibility of feed and access to plant nutrients [4,5,6]. Phytases are enzymes that catalyse the dephosphorylation of phytate in a stepwise manner to degrade inositol phosphate esters (i.e., myo-inositol pentaphosphate to myo-inositol monophosphate). Up to 80% of the total P content in plants may be present in the form of phytate and is effectively unavailable for monogastric or agastric aquatic animals [10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
