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Abstract
A new method for spectral library search based on the dependences of absorbance on the modifier contents in binary mobile phases was developed. The normalisation of spectra by absolute and relative maximum and with respect to the area was tested. The correlation coefficient and the Euclidian distance were used to compare the spectra. The new method of library search allows interpolation of spectra at every composition of eluent in the model range. The range is bracketted by the upper and lower content of modifier in the eluent. In this range five reference spectra were recorded. For validation 34 spectra of pharmaceutical substances in water/methanol and water/acetonitrile mixtures were recorded. In the best case 97 % of spectra were identified correctly. The main problems encountered are that very similar substances e.g. dexa- and betamethasone, have similar spectra as well as similar spectral dependences on the composition of the mobile phase.
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