Abstract
PURPOSE: Determine the effects of oral chronic supplementation with L-glutamine and L-alanine, both in its free form or as dipeptide on gluthatione concentration and lipid peroxidation in rats submitted to heavy resistance training METHODS: Adult male Wistar rats were submitted to resistance training for 6 weeks and supplemented with L-alanyl-L-glutamine (DIP, group), L-glutamine and L-alanine, both in its free form (GLN+ALA, group), L-alanine only (ALA, group) or water (CTRL, group). Resistance training consisted to climb a ladder 3 to 6 sets with progressive loads (25% to 100% of body weight). In the last 21 days of training, supplements were given in a 4% solution dissolved in drinking water. Reduced (GSH) and oxidized (GSSG) gluthatione (mmol/g fresh tissue) GSSG/GSH ratio and TBARS (nmol/mg fresh tissue) were determined in liver and extensor digitorum longus (EDL) muscle. The results were evaluated through the one-way ANOVA and Tukey’s test, with a significance level of 5%. RESULTS: In EDL muscle, a significantly increase of GSH concentration was observed in ALA (0.47±0.05) GLN+ALA (0.50±0.03) and DIP(0.52±0.04) groups compared to CTRL (0.32±0.03). Hence GSSG/GSH ratio (0.054±0.008; 0.038±0.003; 0.039±0.004, respectively) was lower than CTRL (0.091±0.01) (p<0.05). Skeletal muscle lipid peroxidation decreased in ALA (5.0±0.8), GLN+ALA (5.2±0.6) and DIP (4.1±0.8) groups when compared to CTRL (8.26± 0.60) (p<0.05). Liver GSH concentrations were also improved in GLN+ALA (2.85±0.34) and DIP (2.93±0.23) groups compared to CTRL (1,73±0,19). GSSG/GSH ratio decreased in these groups by 34% and 39%, respectively (p<0.05). Liver lipid peroxidation was significantly reduced in GLN+ALA (26%) and DIP (36%) compared to CTRL. CONCLUSIONS: L-glutamine and L-alanine both free or as dipeptide improve glutathione concentration, consequently enhance the redox state and attenuate oxidative stress induced at heavy resistance training. ACKNOWLEDGEMENT: FAPESP
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
