L-glutamate decarboxylase immunoreactivity in developing sympathetic tissues of the rat

Abstract

An indirect immunofluorescence method was used to study the appearance and distribution of l-glutamate decarboxylase (GAD), the enzyme synthesizing γ-aminobutyric acid, in developing rat retroperitoneal sympathetic tissues. GAD immunoreactivity was analyzed in correlation with immunoreactivity to the catecholamine-synthesizing enzyme, tyrosine hydroxylase (TH), in the main retroperitoneal paraganglion, adrenal medullae and abdominal sympathetic ganglia. In different abdominal sympathetic tissues TH-immunoreactive cells first appeared on embryonic days 12.5–14.5, while GAD immunoreactivity was first observed in all these tissues in 14.5-day-old embryos (E 14.5). This suggests that the first expression of GAD is not coupled to the onset of catecholamine synthesis. In developing chain ganglia, GAD immunoreactivity was localized prenatally only in cell clusters with bright TH immunoreactivity, suggesting that GAD is expressed only in the cell lineage leading to ganglionic small intensely fluorescent (SIF) cells. The coeliac-superior mesenteric ganglion complex developed from the preaortic sympathetic tissue, starting from E 16.5 embryos, when the cranial, moderately TH-immunoreactive cells of this tissue were seen to form compact cell islets around the branches of the abdominal aorta. The caudal, intensely TH-immunoreactive cells of preaortic sympathetic tissue were seen to form the main retroperitoneal paraganglion from day E 15.5. During the prenatal period GAD immunoreactivity in preaortic sympathetic tissue was present caudally only in these paraganglionic cells and cranially in some brightly TH-immunoreactive cells, representing SIF and/or paraganglionic cells. In the adrenal medulla, only some of the TH-immunoreactive cells showed GAD immunoreactivity during early developmental stages. The moderately TH-immunoreactive, noradrenaline-synthesizing, cell clusters were seen for the first time in E 16.5 embryos, and they exhibited no GAD immunoreactivity. Thereafter, GAD was expressed only in the intensely TH-immunoreactive, adrenaline-synthesizing, cell clusters. The results of this study indicate that in the developing rat sympathetic tissues GAD is present only in the cell lineages which differentiate into SIF cells of abdominal sympathetic ganglia, preaortic paraganglionic cells and adrenaline cells of the adrenal medulla.