Lewinella litorea sp. nov., isolated from marine sand.

Abstract

A Gram-stain-negative, aerobic, non-motile and ovoid- or rod-shaped bacterial strain, designated HSMS-39T, was isolated from marine sand sampled at Hongsung, Republic of Korea. Strain HSMS-39T grew optimally at 30 °C and in the presence of 1.0-2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain HSMS-39T fell within the clade comprising the type strains of Lewinella species, clustering with the type strain of Lewinella marina showing 16S rRNA gene sequence similarity of 99.1 %. It exhibited 16S rRNA gene sequence similarities of less than 95.5 % to the type strains of the other Lewinella species. Strain HSMS-39T contained MK-7 as the predominant menaquinone and iso-C17 : 1ω9c, iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as the major fatty acids. The major polar lipids of strain HSMS-39T were phosphatidylethanolamine and one unidentified phospholipid. The DNA G+C content of strain HSMS-39T was 60.0 mol%. The mean DNA-DNA relatedness value between strain HSMS-39T and the type strain of L. marina was 15 %. The average nucleotide identity value between strain HSMS-39Tand the type strain of L. marina was 81.87 %. The phylogenetic and genetic data and differential phenotypic properties indicated that strain HSMS-39T is separated from other recognized species of the genus Lewinella. On the basis of the polyphasic data, strain HSMS-39T is considered to represent a novel species of the genus Lewinella, for which the name Lewinella litorea sp. nov. is proposed. The type strain is HSMS-39T (=KACC 19866T=NBRC 113585T).