Leveraging Pseudomonas Stress Response Mechanisms for Industrial Applications.

Kelly Craig,Brant R Johnson,Amy Grunden

doi:10.3389/fmicb.2021.660134

Kelly Craig, Brant R Johnson + Show 1 more

Open Access

https://doi.org/10.3389/fmicb.2021.660134

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Abstract

Members of the genus Pseudomonas are metabolically versatile and capable of adapting to a wide variety of environments. Stress physiology of Pseudomonas strains has been extensively studied because of their biotechnological potential in agriculture as well as their medical importance with regards to pathogenicity and antibiotic resistance. This versatility and scientific relevance led to a substantial amount of information regarding the stress response of a diverse set of species such as Pseudomonas chlororaphis, P. fluorescens, P. putida, P. aeruginosa, and P. syringae. In this review, environmental and industrial stressors including desiccation, heat, and cold stress, are cataloged along with their corresponding mechanisms of survival in Pseudomonas. Mechanisms of survival are grouped by the type of inducing stress with a focus on adaptations such as synthesis of protective substances, biofilm formation, entering a non-culturable state, enlisting chaperones, transcription and translation regulation, and altering membrane composition. The strategies Pseudomonas strains utilize for survival can be leveraged during the development of beneficial strains to increase viability and product efficacy.

Highlights

Members of the genus Pseudomonas have drawn interest for their biotechnology and agricultural potential along with their medical importance as plant and human pathogens
Pseudomonas species are subjected to stress in the natural environment and have adapted mechanisms to survive these harsh conditions
Bacteria have evolved a number of processes aimed at surviving or thriving under stressful conditions

Summary

Introduction

Members of the genus Pseudomonas have drawn interest for their biotechnology and agricultural potential along with their medical importance as plant and human pathogens. Pseudomonas strains have evolved to rapidly respond to the harsh effects of heat stress through production of chaperones and proteases, and regulating with thermosensors and alternative sigma factors (Figure 3). The P. putida KT2442 dnaJ mutant was temperature-sensitive and formed more protein aggregates in response to heat stress compared to the wild type.

Results

Conclusion