Abstract
Testosterone, androstenedione, estrone and estradiol-17β were determined in the testes of fetal sheep ranging in age from 30 to 132 days of intrauterine life. These steroids were measured by micromethods using gas-liquid chromatography with electron capture detection. The presence of testosterone and androstenedione was demonstrated in the fetal testis on the 30th day of gestation, 5 days before onset of sexual differentiation and the earliest period studied. The amounts of testosterone and androstenedione were similar in 46- to 65-day-old testes, while the levels of testosterone were much higher than those of androstenedione at the 30th and 35th days and after the 65th day of intrauterine life. Detectable amounts of estrone were present in 70-day-old testes. (Endocrinology 85: 280, 1969)
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