Levels of phosphate buffered saline- and citrate buffer-elutable immunoglobulins in healthy and inflamed gingiva of dogs.

Abstract

The amounts of phosphate buffered saline (PBS)‐elutable IgG, IgA, and IgM and citrate buffere‐elutable IgG derived from insoluble immune complexes in healthy and inflamed gingiva of dogs were determined. To obtain healthy gingiva, the dogs' teeth were brushed for 6 wk. After achieving clinically healthy gingiva, plaque formation was induced by placement of silk floss ligatures in the cervical area of teeth and inflamed gingiva was obtained after 1 wk and 1 and 3 months of plaque accumulation. The PBS‐elutable IgG was measured by single radial immunodiffusion, IgA and IgM by electroimmunoassay, and citrate buffer‐elutable IgG by enzyme‐linked immunosorbent assay. The PBS‐elutable IgG and IgM in inflamed gingiva showed a tendency to increase with the progress of the disease. Particularly, the amount of IgG after 3 months of plaque formation increased to 183.2 μg/100 μg DNA and was significantly higher than that of the healthy gingiva, 88.2 μg/100 μg DNA (p < 0.05). The IgM value was highest 1 wk after plaque accumulation. On the other hand, the amount of citrate buffere‐lutable IgG in inflamed gingiva was significantly higher than that in healthy gingiva at all experimental periods (p < 0.05) and the increase showed a close parallel with the progression of inflammation.