Abstract
The levels of essential and non-essential metals were determined in linseed (Linum usitatissimum) samples collected in November 2011 from five different sites (Bale, East Gojam, Shoa, South Wello and Tigray) in Ethiopia where its cultivation is common. A 0.5 g dried powdered linseed was digested with 2 mL of nitric acid (HNO3), 1 mL of perchloric acid (HClO4) and 1 mL of hydrogen peroxide (H2O2) at 270 oC for 2:30 hours and the levels of metals determined by flame atomic absorption spectrometer. The accuracy of the optimized procedure was evaluated by analyzing the digest of the spiked samples with standard solution. Recoveries of the spiked samples varied from 91% to 109%. The levels (mg kg-1) of metals determined were in the ranges Na (242–614), K (6,494–6,755), Mg (2,679–3,118), Ca (540–744), Cr (13–30), Mn (17–28), Fe (198–242), Co (23–42), Ni (12–16), Cu (25–45), Zn (29–40), and Pb (12–32). Cd was below method detection limit. K and Fe were with the highest concentration from major and trace metals, respectively. Analysis of variance (ANOVA) at 95% confidence level indicated that there is significant difference in the levels of all metals between the five samples means except K and Ni. The results indicated that Ethiopian linseed is a good source of essential metals and free from the toxic metal Cd but not from Pb. KEY WORDS: Linseed, Linum usitatissimum, Essential metals, Non-essential metals, Ethiopia Bull. Chem. Soc. Ethiop. 2014, 28(3), 349-362.DOI: http://dx.doi.org/10.4314/bcse.v28i3.4
Highlights
Linseed (Linum usitatissimum) is an erect annual, forming a short taproot with fibrous branches which may extend 90-120 cm in light soils
The principal linseed growing regions in Ethiopia are located at altitudes between 1800 and 2800 meter above sea level, it occasionally grows at altitudes as low as 1680 masl or as high as 3430 masl [4]
The determination of metals level was carried out by FAAS and the accuracy and precision of the results was determined by different statistical methods as described in experimental section
Summary
Linseed (Linum usitatissimum) is an erect annual, forming a short taproot with fibrous branches which may extend 90-120 cm in light soils. In another study conducted on some medicinal plants being used as food condiments including linseed in South Asian region, their composition by different methods and metals content by FAAS have been determined [15].
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