Abstract
The question regarding clarification the mechanisms that link changes in the musculoskeletal system with changes in the microbiome, in recent few years has become particularly relevant. The investigation of the biological effect of probiotics on cartilage metabolism under osteoarthritis (OA) opens the perspectives for their use in complex therapy and prevention of joint pathology. The aim of the research was to analyze the quantitative and qualitative composition of the peptide component of the middle-mass molecules (MMM) of different fractions in the articular cartilage of rats with experimental OA under the introduction of multiprobiotic (PB) composition. The experiments were conducted on white male non-linear rats weighing 180-200 g. Experimental osteoarthritis in rats was induced by a single injection of sodium monoiodacetate (MIA; Sigma, USA) in the knee patellar ligament. The animals of therapeutic group received oral administration of live probiotic composition Multiprobiotic Simbiter® acidophilic concentrated ("O.D. Prolisok", Ukraine) at a dose of 140 mg/kg daily for 14 days. Euthanasia of animals was performed on the 30th day of the experiment. The level of MMM was assessed spectrophotometrically. Fractionation at the peptide level was performed using the method of chromatography, which is separated by size on Sephadex G 15 column. Our findings showed an increase in the MMM content of all studied fractions in cartilage tissue of rats with experimental OA and changes in the qualitative and quantitative composition of their the peptide component, that could indicate the development of endogenous intoxication, as a result of impaired cartilage metabolism, inflammation and destructive processes in the knee joint during the pathology. Administration to animals with MIA-induced OA PB composition had a favorable effect on the studied parameters, which was expressed in a decrease of MMM content and restored redistribution of the peptide pool of cartilage tissue. The obtained results provide grounds for further research aimed at studying the biological effect of PB on cartilage metabolism, which may contribute to the development of new strategies for the treatment and prevention of joint diseases.
Highlights
Osteoarthritis (OA) is the most common disease among musculoskeletal pathologies, mainly among middle-aged and elderly people, and occupies a leading place among the global causes of disability [1]
Under conditions of chronic endogenous intoxication, articular cartilage becomes a target for endogenous toxic compounds and the systemic effects mediated by them, in particular, mediators of the cytokine cascade, which results in disruption of the process of physiological regeneration and remodeling of cartilage tissue
Articular cartilage is specialized connective tissue which unlike most tissues, does not have blood or lymphatic vessels and nerves. It is composed of a dense extracellular matrix (ECM), the main components of which are macromolecules of collagen and proteoglycans (PG), including aggrecan, decorin, biglycan, and fibromodulin, with other noncollagenous proteins and glycoproteins present in lesser amounts which essential for normal function
Summary
Osteoarthritis (OA) is the most common disease among musculoskeletal pathologies, mainly among middle-aged and elderly people, and occupies a leading place among the global causes of disability [1]. The OA is considered as a whole organ disease in which all components of the joint are involved in the pathological process: cartilage, subchondral bone, synovial membrane, ligaments, capsules and periarticular muscles. Endogenous intoxication (EI), which is associated with the accumulation in tissues and biological fluids of the organism of excess metabolites of normal and pathological metabolism, waste products of different pathogenic microorganisms is observed in various, etiologically and pathogenetically dissimilar conditions [8]. Under conditions of chronic endogenous intoxication, articular cartilage becomes a target for endogenous toxic compounds and the systemic effects mediated by them, in particular, mediators of the cytokine cascade, which results in disruption of the process of physiological regeneration and remodeling of cartilage tissue
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
