Level of ATP synthase activity required for yeast Saccharomyces cerevisiae to grow on glycerol media

Abstract

Two independent cold-sensitive pet mutants in the gene (ATP5) coding for the oligomycin sensitivity conferring protein (OSCP) have been isolated in the yeast Saccharomyces cerevisiae. The mutations in both strains alter the initiating methionine codon in the ATP5 gene: ATG to ATA (Ile) and AAG (Lys). Western blot analysis of total yeast protein after the cells were grown at 18°C, 30°C, and 37°C, indicates that the level of OSCP decreased 80% relative to the wild type strain. In addition, the level of the oligomycin-sensitive ATPase decreased 85% relative to the wild type strain, after growth at 30°C. These findings indicate that for S. cerevisiae, the level of oxidative phosphorylation can decrease 85% without showing a large growth defect on media containing glycerol at 30°C, but not at 18°C.