Abstract
Levansucrase (EC 2.4.1.10) from Zymomonasmobilis was purified from protamine sulfate treated cell free extracts by DEAE-trisacryl ionexchange chromatography to produce a 330-fold partial purification with an 11 % yield. Levansucrase was inhibited completely by glucose (30mM) and ethanol (1.6 M). These concentrations of glucose and ethanol were exceeded by cells growing in cultures containing an initial sucrose concentration of 200 gl−1.
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