Abstract
12 halophilic strains from different hypersaline environments such as solar salterns in Tuzlagözü (Sivas), Fadlum (Sivas), Kemah (Erzincan), a hypersaline spring water in Pülümür (Tunceli) and a saline lake in Delice (Kırıkkale) belonging to Turkey, were investigated in terms of levan production. After incubation and ethyl alcohol treatment, dialysis process was operated for partial purification. Levan amounts in our samples after hydrolysis were calculated based on the amount of sugar obtained by acid hydrolysis of standard levan. Sugar amount of samples were determined using by high performance liquid chromatography system (HPLC). 1H-Nuclear Magnetic Resonance (1H-NMR) spectra of the levan sample and standard were recorded. The results obtained by HPLC analysis showed that Chromohalobacter canadensis strain 85B had highest production potential as 234.67 mg levan/g biomass. The chemical shifts of 1H-NMR spectrum of the extracted levan also showed high similarity to those of pure levan isolated from Erwinia herbicola. Furthermore, Marinobacter sp. 163Y strain also is also capable with regard to levan production. In this study, this strain could yield 230.80 mg/g levan and this potential was first reported in the literature.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of microbiology, biotechnology and food sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
