Leukotriene mechanisms of allergic enterocolitis pathogenesis in young children

Abstract

Purpose of the study was to determine the parameters of lipid mediators of allergic inflammation (cysteinyl leukotrienes C4, D4, E4) by enzyme immunoassay in serum, saliva, urine of young children with allergic enterocolitis. Materials and methods. 40 patients aged from 3 months to 3 years (mean age - (19.9 ± 2.7) months) were examined, among them 20 children with allergic enterocolitis, 10 patients with asthma at the acute stage and 10 healthy babies. Clinical, allergological, endoscopic examination and concentrations of cysteinyl leukotrienes (LTB4, LTC4, LTE4) determination in the biological fluids (blood serum, saliva, urine) using the enzyme immunoassay kits (Enzo Life Science, USA) on a Multiskan photometer Plus “Labsystems” were conducted. The obtained data results were processed statistically. Significance of differences was assessed using Student's t-test and Kendall's tau rank correlation analysis. Difference with a p value 0.05), blood and saliva τ = 0.07 (p>0.05), urine and saliva τ = -0.52 (P > 0.05). Conclusions. An increase in cysteinyl leukotrienes concentration in blood serum, urine, saliva was found in young children with allergic enterocolitis in comparison with the control group. The absence of a significant rank correlation between leukotrienes concentration in the blood and urine, blood and saliva, saliva and urine indicates that any biological fluid, in particular saliva or urine, could be selected as a non-invasive method for determination of leukotrienes concentration to monitor the activity of allergic inflammation.