Abstract
The epoxide 5(S) trans-5,6 oxido, 7,9 trans-11,14,17 cis eicosatetraenoic acid (leukotriene A5) was chemically synthesized and demonstrated to be both a substrate and an inhibitor of partially purified rat and human LTA4 hydrolase. Both rat and human LTA4 hydrolase utilized leukotriene A5 less effectively as a substrate than leukotriene A4. Incubation of leukotriene A5 (10 μM) or leukotriene A4 (10 μM) with rat neutrophils demonstrated formation of 123 pmol LTB5/min/107 cells and 408 pmol LTB4/min/107 cells respectively. Purified rat neutrophil LTA4 hydrolase incubated with 100 μM leukotriene A5 produced 22 nmol LTB5/min/mg protein and when incubated with 100 μM leukotriene A4 produced 50 nmol LTB4/min/mg protein. Human neutrophil LTA4 hydrolase incubated with 100 μM leukotriene A5 produced 24 nmol LTB5/min/mg protein and when incubated with 100 μM leukotriene A4 produced 52 nmol LTB4/min/mg protein. Leukotriene A5 was an inhibitor of the formation of leukotriene B4 from leukotriene A4 by both the rat and human neutrophil LTA4 hydrolase. Excess leukotriene A5 prevented covalent coupling of [3H] leukotriene A4 to LTA4 hydrolase suggesting inhibition may involve covalent coupling of leukotriene A5 to the LTA4 hydrolase.
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