Leukocyte-Poor Platelet Concentrates at the Bedside by Filtration through Sepacell-PL

Abstract

We developed a new filter, ‘Sepacell-PL’, consisting of a blood administraiton set for the preparation of leukocyte-poor platelet concentrates at the patient’s bedside. This filter is packed with 1.8 pm polyester fibers coated with a new polymer, and has a volume of 14 ml. Sepacell-PL removed more than 99% of the leukocytes while approximately 93% of platelets were recovered from 10 units of platelet concentrates which contained 5 x 10^8 leukocytes and 3 x 10^11 platelets. The functions of pre- and postfiltrated platelets were unchanged. The filtration procedure is very simple, without priming with saline to remove air and wet the filter. Moreover, platelet concentrates can be passed through the filter at a flow rate of 4 ml/min, or at a higher flow rate of 54 ml/min by gravity.