Leukocyte recognition with convolutional neural network

Abstract

Accurate segmentation of leukocytes is a primary and very difficult problem because of the non-uniform color, uneven illumination of blood smear image. An improved algorithm based on feature weight adaptive K-means clustering for extracting complex leukocytes is proposed. In this paper, the initial clustering center is chosen according to the histogram distribution of a cell image; this approach not only improves the clustering effect but also reduces the time complexity of the algorithm from O (n) to O (1). Prior to white blood cell extraction, the color space is decomposed. Then, color space decomposition and K-means clustering are combined for image segmentation. And then adherent complex white blood cells are separated again based on watershed algorithm. Finally, classification experiments based on convolutional neural network were performed and compared with other methods; 368 representative images were used to evaluate the performance of our method. The proposed segmentation method achieves 95.81% segmentation accuracy. The classification accuracy reached a maximum of 98.96%, and the average classification time is 0.39 s. Compared with those in the existing algorithms for WBC, convolutional neural network classification method not only presents obvious advantages but can also be easily improved.