Leukocyte reaction of peripheral blood in secondary chronic carrageenan inflammation after administration of the thrombin blocker dabigatran etexilate

Abstract

The experimental study was conducted on 72 adult male WAG laboratory rats weighing 180–200 g. The study was conducted in accordance with the guidelines of Directive 2010/63/EU of the European Parliament and of the Council on the protection of animals used for scientific purposes. Intact rats served as a control for the natural course of inflammation, and rats receiving dabigatran etexilate without further induction of chronic inflammation served as a control for inflammation in the setting of dabigatran etexilate administration. The model of inflammation was carrageenan secondary chronic aseptic inflammation, which was induced by intramuscular injection of 10 mg λ-carrageenan (Sigma, USA) dissolved in 1 ml of isotonic sodium chloride solution into the right thigh. Dabigatran etexilate was administered intragastrically through a gavage tube at a dose of 15 mg/kg/day dissolved in 1 ml of isotonic sodium chloride solution daily throughout the experiment, which lasted 28 days. The results of the study have shown that the use of a thrombin blocker dabigatran etexilate, compared with the natural course of secondary chronic carrageenan inflammation, promotes the involvement of leukocytes on the 14th day, which leads to greater elimination of phlogogen during this period, and a decrease in the number of leukocytes on the 21st and 28th days during the period of chronic inflammation, which indicates a decrease in the need for leukocytes in these periods. The tendency to increase the number of segmented neutrophils by 1.3 times on the 28th day of inflammation after administration of dabigatran etexilate, compared with the natural course of inflammation, indicates a less pronounced emigration of leukocytes to the inflammatory focus due to a decrease in the intensity of chronic inflammation. A decrease in the number of banded neutrophils in the peripheral blood on the 28th day may be associated with a decrease in their emigration to the inflammatory focus. A significant decrease in the number of monocytes by 1.62 times (p<0.01) on the 28th day indicates that the use of the thrombin blocker dabigatran etexilate reduces leukocyte adhesion and reduces the chronicity of inflammation. Keywords: toxic action, biochemical variant, antimicrobials.