Abstract
Intense metabolism and fast exhaustion of the energy potential of nucleate blood cells at positive temperatures substantially reduce the duration of their storage in a viable state. However, this transfusion environment is extremely necessary for clinical purposes. The popular method of cell preservation at an ultralow temperature (−196°C) is limited in application because of the use of liquid nitrogen, special equipment, and trained engineering staff. The goal of this study was to develop an efficient and simple method of leukocyte preservation at near-zero temperatures (+2°–0°C) using a standard household refrigerator and a novel cryopreservation solution.
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