Leukocyte integrin expression in patients undergoing cardiopulmonary bypass

Abstract

Background. The recruitment of leukocytes to vascular endothelium is controlled by adhesion events mediated through the β2 integrins, whereas the response of extravasated leukocytes within the tissues is controlled through the β1 integrins. Although cardiopulmonary bypass (CPB) has been shown to be associated with a systemic inflammatory response and elevated levels of β2 integrins on leukocytes, its effect on the β1 integrins is not known. This study investigated the effect of the protease inhibitor aprotinin on the expression of the β1 and β2 integrins on circulating leukocytes in patients undergoing CPB.Methods. Patients undergoing primary elective coronary artery bypass grafting were randomized into full-dose aprotinin or placebo groups. Blood samples were obtained at nine time points preoperatively, intraoperatively, and up to 6 days postoperatively. The surface expression of the β1 integrins VLA-1, -3, -4, -5, and -6 and of the β2 integrins CD11a/CD18, CD11b/CD18, and CD11c/CD18 was measured by flow cytometry on gated neutrophil and monocyte subpopulations in whole blood.Results. Expression of the β1 integrins was not significantly altered during the study period and, therefore, aprotinin had no effect on the expression of these molecules. Of the β2 integrins, CD11b/CD18 expression was significantly increased on neutrophils at 15 minutes after onset of CPB in the placebo group (p < 0.01) but not in the aprotinin group.Conclusions. This study showed that expression of the β1 integrins on neutrophils and monocytes did not alter during the first 6 days after CPB. Expression of the β2 integrin CD11b/CD18 increased significantly on neutrophils during CPB in control patients but not in patients treated with full-dose aprotinin.