Abstract
One of the main differences among platelet-rich plasma (PRP) products is the inclusion of leukocytes that may affect the biological efficacy of these autologous preparations. The purpose of this study was to evaluate whether the addition of leukocytes modified the morphological, biomechanical and biological properties of PRP under normal and inflammatory conditions. The release of pro-inflammatory cytokines from plasma rich in growth factors (PRGF) and leukocyte-platelet rich plasma (L-PRP) scaffolds was determined by enzyme-linked immunosorbent assay (ELISA) and was significantly increased under an inflammatory condition when leukocytes were included in the PRP. Fibroblasts and osteoblasts treated with L-PRP, under an inflammatory situation, underwent a greater activation of NFĸB pathway, proliferated significantly less and secreted a higher concentration of pro-inflammatory cytokines. These cellular events were assessed through Western blot and fluorimetric and ELISA methods, respectively. Therefore, the inclusion of leukocytes induced significantly higher pro-inflammatory conditions.
Highlights
Over the last few years, translational medicine has emerged as a new trend in medical practice, which aims to promote the rapid clinical translation of a wide range of therapeutic options and to receive the feedback of basic research results [1]
Leukocyte concentration in L-platelet-rich plasma (PRP) was 3.1 ± 0.3-fold higher than in blood while almost no white cell was detected in plasma rich in growth factors (PRGF) (0.2 ± 0.1 x103/μl) (Fig 2B)
PRGF fibrin scaffold was yellowish and easier to handle than the reddish one prepared from leukocyte-platelet rich plasma (L-PRP) (Fig 2C)
Summary
Over the last few years, translational medicine has emerged as a new trend in medical practice, which aims to promote the rapid clinical translation of a wide range of therapeutic options and to receive the feedback of basic research results [1]. Restoration and healing of lost tissue are unique among clinical treatments due to the large number of patients suffering from tissue damage and injuries. Progress in this field is pertinent as it may help to reduce the burden on the world’s health systems and address the need of tissue replacement [3]. Human plasma and especially human platelets contain a wide range of PLOS ONE | DOI:10.1371/journal.pone.0121713 March 30, 2015
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