Leukocyte Cell Population Analysis From the Coulter Automatic Blood Cell Analyzer DxH800 to Monitor the Effect of G‐CSF

Abstract

Granulocyte colony-stimulating factor (G-CSF) induces the formation of toxic granulation neutrophils (TGNs), which are found in many inflammatory responses. Cell population data (CPD) may be able to clarify the effect of G-CSF, and potentially help doctors in discriminating the effect of G-CSF from other inflammatory situations. To achieve this, we performed analyses of leukocyte CPD from normal controls and healthy donors that had received G-CSF for peripheral blood stem cells (PBSCs) mobilization (G-CSF group). Two hundred and seventy-one subjects were enrolled as normal controls, and 21 subjects were enrolled in the G-CSF group. Mean volume (MN-V)-neutrophils (NE), mean axial light loss (MN-AL2)-NE, and all standard deviation (SD) parameters increased significantly, whereas all light scattering parameters, mean median angle light scatter (MN-MALS)-NE, mean upper median angle light scatter (MN-UMALS)-NE, mean lower median angle light scatter (MN-LMALS)-NE, and mean low angle light scatter (MN-LALS)-NE reduced significantly in the G-CSF group. MN-V-lymphocytes (LY) from the G-CSF group showed no significant difference (P = 0.143), whereas MN-V-monocytes (MO) were significantly decreased (P < 0.001). Receiver operating characteristic (ROC) curves for the discrimination of the G-CSF group from normal controls showed excellent sensitivity in SD-LALS-NE (at 30.85, sensitivity 95.2%, specificity 76.0%), MN-AL2-NE (at 134.5, sensitivity 90.5%, specificity 83.0%), and SD-AL2-NE (at 16.4, sensitivity 95.2%, specificity 95.2). Several CPD parameters of lymphocytes and monocytes, as well as neutrophils can be used as markers for determining the effect of G-CSF. Our data show that many CPD of leukocytes can be considered to be useful parameters of the effect of G-CSF.