Abstract
The serum-free mouse embryo (SFME) cell line, derived in serum-free medium from 16-day-old mouse embryos, exhibit unique properties. SFME cells grow indefinitely in culture without senescence, require epidermal growth factor (EGF) or fibroblast growth factor (FGF) for survival and are growth inhibited by serum. The cell line expresses glial fibrillary acidic protein (GFAP) in response to transforming growth factor β (TGF β) or serum and cells with similar properties can be isolated directly from brain. Culture of SFME cells with leukemia inhibitory factor (LIF), a peptide implicated in neural tissue development, also resulted in expression of GFAP. Other peptides that share signal transduction mechanism with LIF — ciliary neurotrophic factor (CNTF), oncostatin M (OSM) and interleukin-6 (IL-6) — also caused expression of GFAP in the cells. These effects were inhibited by concentrations of EGF or FGF that promoted rapid cell growth.
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