Abstract
Background: Platelet-rich plasma (PRP) is an autologous blood product that contains a high concentration of platelets and leucocytes, which are fundamental fibroblast proliferation agents. Literature has emerged that offers contradictory findings about leucocytes within PRP. Herein, we elucidated the effects of highly concentrated leucocytes and platelets on human fibroblasts. Methods: Leucocyte-rich, PRP (LR-PRP) and leucocyte-poor, platelet-poor plasma (LP-PPP) were compared to identify their effects on human fibroblasts, including cell proliferation, wound healing and extracellular matrix and adhesion molecule gene expressions. Results: The LR-PRP exhibited 1422.00 ± 317.21 × 103 platelets/µL and 16.36 ± 2.08 × 103 white blood cells/µL whilst the LP-PPP demonstrated lower concentrations of 55.33 ± 10.13 × 103 platelets/µL and 0.8 ± 0.02 × 103 white blood cells/µL. LR-PRP enhanced fibroblast cell proliferation and cell migration, and demonstrated either upregulation or down-regulation gene expression profile of the extracellular matrix and adhesion molecules. Conclusion: LR-PRP has a continuous stimulatory anabolic and ergogenic effect on human fibroblast cells.
Highlights
Platelet-rich plasma (PRP) has gained encouraging recognition as an accepted protocol for treating injuries; research has mainly focused on orthobiologic applications with few studies being performed on fibroblasts and their relation to cutaneous delayed wound healing [1,2,3,4,5,6]
Haematocrit percentages of the initial collected blood, LR-PRP and LP-PPP volume were calculated by applying the formulae that are available in Supplementary Materials (Table S2) [18,43]
The present study provides indications of the ability of LR-PRP and LP-PPP to induce challenged fibroblasts in vitro
Summary
Platelet-rich plasma (PRP) has gained encouraging recognition as an accepted protocol for treating injuries; research has mainly focused on orthobiologic applications with few studies being performed on fibroblasts and their relation to cutaneous delayed wound healing [1,2,3,4,5,6]. This study engaged in three in vitro experiments to elucidate the effects of LR-PRP on challenged fibroblasts, as follows: (i) fibroblast proliferation assay; (ii) scratch wound assay; and (iii) the human extracellular matrix (ECM) and adhesion molecules RT2 Profiler PCR gene array. Platelet-rich plasma (PRP) is an autologous blood product that contains a high concentration of platelets and leucocytes, which are fundamental fibroblast proliferation agents. Methods: Leucocyte-rich, PRP (LR-PRP) and leucocyte-poor, platelet-poor plasma (LP-PPP) were compared to identify their effects on human fibroblasts, including cell proliferation, wound healing and extracellular matrix and adhesion molecule gene expressions. LR-PRP enhanced fibroblast cell proliferation and cell migration, and demonstrated either upregulation or down-regulation gene expression profile of the extracellular matrix and adhesion molecules. Conclusion: LR-PRP has a continuous stimulatory anabolic and ergogenic effect on human fibroblast cells
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