Abstract
AbstractA simple method is described to assay for factors that mobilize leucocytes in the bone marrow. Guinea pig or rat femura were slowly perfused with Medium 199 and the cell concentration in the effluent was determined. Leucocyte mobilizing factor (LMF) was recognized by an increase in the effluent cell concentration following addition of the material to the perfusion medium.By reaction with C 142, the rabbit C3 molecule was cleaved, releasing a fragment with LMF activity. A purification procedure is described. The factor was devoid of chemotactic activity. It was negatively charged at pH 8.6.LMF may be involved in the pathogenesis of the leucocytosis associated with C dependent immune reactions in vivo.
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