Abstract
Significant depots of brown adipose tissue (BAT) have been identified in many adult humans through positron emission tomography (PET), with the amount of BAT being inversely correlated with obesity. As dietary activation of BAT has implications for whole body glucose metabolism, leucine was used in the present study to determine its ability to promote BAT activation resulting in increased glucose uptake. In order to assess this, 2-deoxy-2-(fluorine-18)fluoro-d-glucose (18F-FDG) uptake was measured in C57BL/6 mice using microPET after treatment with leucine, glucose, or both in interscapular BAT (IBAT). Pretreatment with propranolol (PRP) was used to determine the role of β-adrenergic activation in glucose and leucine-mediated 18F-FDG uptake. Analysis of maximum standardized uptake values (SUVMAX) determined that glucose administration increased 18F-FDG uptake in IBAT by 25.3%. While leucine did not promote 18F-FDG uptake alone, it did potentiate glucose-mediated 18F-FDG uptake, increasing 18F-FDG uptake in IBAT by 22.5%, compared to glucose alone. Pretreatment with PRP prevented the increase in IBAT 18F-FDG uptake following the combination of glucose and leucine administration. These data suggest that leucine is effective in promoting BAT 18F-FDG uptake through β-adrenergic activation in combination with glucose.
Highlights
Diabetes continues to climb the list of the top ten causes of death world wide [1], placing an ever-increasing personal and financial burden on society
In order to determine the ability to stimulate interscapular Brown adipose tissue (BAT) (IBAT) glucose uptake, 2g/kg glucose was administered with the 18 F-FDG
We demonstrate that leucine can potentiate glucose-induced 18 F-FDG uptake in IBAT
Summary
Diabetes continues to climb the list of the top ten causes of death world wide [1], placing an ever-increasing personal and financial burden on society. Diabetes Atlas states that type 2 diabetes (T2D) is the most prevalent form of the disease [2], with up to. 91% of adults with diabetes in high-income countries presenting with this type. Despite developments in prevention of T2D, the number of affected adults continues to increase, with even more adults suffering from impaired glucose tolerance putting them at a high risk of developing the disease in the future. Brown adipose tissue (BAT), once thought to only be relevant in human infants for promoting thermogenesis, has more recently been identified in adults as having the ability to promote whole body glucose metabolism [3].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
