Abstract
Recent studies have found various compounds that can extend lifespan in different species. Amino acids as regulators in nutrition process and anti-aging have been investigated, but inconsistency existed in the literature in the context of lifespan-extending roles of some amino acids in C. elegans. In this paper, we measured the effects of individual branched-chain amino acids (BCAAs, leucine, valine and isoleucine) on lifespan in C. elegans. We found that 1000μM and 10000μM leucine could extend lifespan significantly coupled with increased stress resistance of thermotolerance, anti-oxidation and anti- UV irradiation. Furthermore, we used daf-2 and daf-16 mutants to explore the possible molecular mechanism of Leu-induced lifespan extension. Results suggested that the function of Leu on aging regulation is dependent on Insulin/IGF-1 (IIS) signaling. Our work confirmed that BCAAs play an important role in IIS signaling pathway to regulate aging and intake of such nutrients may also be good for healthspan in C. elegans.
Highlights
Aging can be regarded as degeneration of organisms to maintain normal functions in late days, which increases risk of diseases and death
A series of papers have reported that intake of several compounds, included ethanol, metformin and D-allulose, could extend lifespan significantly in C. elegans through different mechanisms [13,14,15]
We checked the effect of BCAAs on lifespan in wild-type C. elegans at different concentrations
Summary
Aging can be regarded as degeneration of organisms to maintain normal functions in late days, which increases risk of diseases and death. Thanks to advanced knowledge in aging research, several age-related phenotypes and longevity regulators have been identified. Since C. elegans was widely used as laboratory research organism, a number of compounds and signaling pathways have been demonstrated to regulate lifespan significantly [1]. Regulatory mechanism of aging is complex, coupled by genes and external environment simultaneously. Some papers have discovered classical signaling pathways that could regulate lifespan in short-lived animals including C. elegans and Drosophila and confirmed the similar effects on mammals [2]. Recent aging research focuses on screening the effect of chemicals and drugs on aging. A series of papers have reported that intake of several compounds, included ethanol, metformin and D-allulose, could extend lifespan significantly in C. elegans through different mechanisms [13,14,15]
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