Abstract
Abstract Western X-disease virus (WXV), when injected into the leafhopper Colladonus montanus (Van Duzee) by means of infectious hemolymph or dilutions of whole insect extracts, caused premature death. Mortality due to virus occurred earlier in injected insects than in those acquiring virus by feeding. Moreover, the position of survival curves with respect to the time scale proved to be a function of the concentration of virus injected. Factors other than virus, such as contaminating bacteria and wounding, were also found to cause premature death of leafhoppers after injection. Deaths due to virus did not begin to appear until about 35 days after injection, and continued until about 70–80 days. Deaths due to WXV were independent of deaths resulting from bacteria. Bacterial contaminants could be eliminated by repeated centrifugation or by means of antibiotics.
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