Lethal Effects ofAspergillus nigeragainst Mosquitoes Vector of Filaria, Malaria, and Dengue: A Liquid Mycoadulticide

Gavendra Singh,Soam Prakash

doi:10.1100/2012/603984

Abstract

Aspergillus niger is a fungus of the genus Aspergillus. It has caused a disease called black mold on certain fruits and vegetables. The culture filtrates released from the A. niger ATCC 66566 were grown in Czapek dox broth (CDB) then filtered with flash chromatograph and were used for the bioassay after a growth of thirty days. The result demonstrated these mortalities with LC50, LC90, and LC99 values of Culex quinquefasciatus 0.76, 3.06, and 4.75, Anopheles stephensi 1.43, 3.2, and 3.86, and Aedes aegypti 1.43, 2.2, and 4.1 μl/cm2, after exposure of seven hours. We have calculated significant LT90 values of Cx. quinquefasciatus 4.5, An. stephensi 3.54, and Ae. aegypti 6.0 hrs, respectively. This liquid spray of fungal culture isolate of A. niger can reduce malaria, dengue, and filarial transmission. These results significantly support broadening the current vector control paradigm beyond chemical adulticides.

Highlights

Emerging insecticide resistance is creating an urgent need for new active ingredients to control the adult mosquitoes [1]
We have reported the lethal effects of purified culture filtrates of A. niger against An. stephensi, Cx. quinquefasciatus, and Ae. aegypti in the laboratory
The colonies of A. niger were grown on Czapek dox agar (CDA), solid medium plates were transferred to each flask using an inoculation needle

Summary

Introduction

Emerging insecticide resistance is creating an urgent need for new active ingredients to control the adult mosquitoes [1]. We have reported the lethal effects of purified culture filtrates of A. niger against An. stephensi, Cx. quinquefasciatus, and Ae. aegypti in the laboratory. The flash chromatograph purified culture filtrates were used for bioassays against laboratory reared Cx. quinquefasciatus, Ae. aegypti, and An. stephensi as per the standard procedures recommended by World Health Organization with some modifications [16].

Results

Conclusion