Lethal effect of leachates from tyre wear particles on marine copepods

Abstract

With thousands of tons of Tyre Wear Particles (TWP) entering the aquatic environment every year, TWP are considered a major contributor to microplastic pollution. TWP leach organic compounds and metals in water, potentially affecting the marine food web. However, little is known about the toxicity of TWP leachates on marine copepods, a major food web constituent, and a key group to determine the environmental risk of pollution in marine ecosystems. In this study, we determined the lethal effect of TWP leachates on marine copepods after 24, 48, and 72-h of exposure to 0.05–100% leachate solutions prepared using a concentration of 5 g TWP L−1. The calanoids Acartia tonsa, Temora longicornis and Centropages hamatus, the cyclopoid Oithona davisae and the harpacticoid Amonardia normanni were used as experimental species. TWP leachates were toxic to all the studied species, with toxicity increasing as leachate solution and exposure time increased. Median lethal concentration (LC50, 72-h) ranged from 0.22 to 3.43 g L−1 and calanoid copepods were more sensitive to TWP leachates than the cyclopoid O. davisae and the harpacticoid A. normanni. Toxicity of TWP leachates was not related to the copepod body size, which suggests that other traits such as foraging behaviour or adaptation to contaminants could explain the higher tolerance of cyclopoid and harpacticoid to TWP leachates compared to calanoid copepods. Although field data on the concentration of TWP and their chemical additives are still limited, our results suggest that TWP leachates can negatively impact planktonic food webs in coastal areas after road runoff events.