Abstract
Ultraviolet-A (365 nm, 120 kJ/m2/h) exposure caused cell death in Pseudomonas aeruginosa at doses at which Escherichia coli cell viability was not affected. We have not found that UVA induced growth delay or any other sublethal effect. Irradiated suspensions of P. aeruginosa showed a marked reduction in membrane-bound succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH) activities. Succinate-driven respiration and several nutrient transport systems were also inhibited. Whereas SDH and LDH activities were independent of the irradiation conditions, cell viability, respiration and transport systems were protected when irradiation was performed in an N2 atmosphere. A similar protective effect was observed when cells were grown in media containing glycerol or when preirradiation bacterial growth was carried out at 30 degrees C (instead of 37 degrees C). Results suggest that UVA induces a differential damaging effect on several biochemical functions of P. aeruginosa. The UVA- induced photodamage may fall into two categories: indirect damage mediated by oxygen (cell killing and inhibition of respiration and transport systems) and direct damage to SDH and LDH (apparently not oxygen dependent). These enzymes and leucine transport appear not to be involved in the lethal effect described herein because they were altered despite viability-preserving conditions
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