Lethal and behavioral sublethal side effects of thiamethoxam on the predator Harmonia axyridis

Abstract

AbstractThiamethoxam (TMX) is one of the most effective neonicotinoid insecticides for the control of green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), and various side effects can be expected in its natural enemies. The multicolored Asian lady beetle or harlequin ladybird, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), is an important predator against M. persicae in greenhouses and fields. In this study, we evaluated the toxicity of TMX to H. axyridis and the effect on the functional response of this predator to M. persicae through three routes of exposure. Acute toxicity bioassays indicated that LC50 values of TMX on H. axyridis through direct residual contact (‘contact’), systemic application (‘systemic’), and leaf‐dip treatment (‘leaf‐dip’) were 18.99, 21.26, and 15.39 mg a.i. l−1, respectively. The hazard quotient indicated a potential hazard of this agrochemical regardless of the exposure routes. The mortality caused by the lowest rate, 2 mg a.i. l−1, was not significantly different compared with the control group. For the three routes of sublethal TMX exposure, the type‐II functional response was a good fit to the prey consumption of H. axyridis. Predation was most affected by leaf‐dip treatment, followed by contact and systemic treatments, which had similar effect. For all exposure routes, the predation capacity of the predator recovered quickly after transfer to untreated cabbage leaves. Thiamethoxam applied systemically was the least toxic to H. axyridis and did not affect the functional response of the predator. However, the sublethal effects of TMX through both contact and leaf‐dip application may reduce the population growth of H. axyridis and consequently impair the biological control of M. persicae by this predator. These results illustrate that the assessment of potential effects of TMX on H. axyridis is crucial to develop effective integrated pest management programs for M. persicae in China.