Abstract
Less tumorigenic mutants of adenovirus 12 were isolated and tentatively named lt mutants. Comparative studies of the mutants with the tumorigenic wild type revealed that the mutants were not different from the wild type in virion structure (antigenicity, polypeptides, heat stability), viral replication, T antigen-forming capacity, cell killing activity, induction of cellular DNA synthesis and integration of viral DNA into cellular DNA. The mutants formed larger plagues, liberated a larger amount of virus into culture medium than the wild type, and produced an intensified cytopathic effect, resembling the cyt mutants of Takemori et al. (1968) in this respect. While the concentration of lectin required to agglutinate mutant-infected cells was not significantly different from that required for uninfected cells, cells infected with the wild type agglutinated in the presence of a lower concentration of lectin. It was shown that the mutants were ineffective in inducing transplantation immunity. These observations suggest that the mutants are defective in inducing the cell-surface change, which might be necessary for transformation of cells but not for viral replication.
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