Abstract
Blood culture is an essential analysis for the diagnosis and the treatment of sepsis. Microorganisms fund in blood cultures can be isolated from community-acquired infections or from hospital infections. In case of sepsis, the physician must give the microbiologist the clinical information, so that the laboratory makes the right choices in term of method, medium and incubation time. Automated blood culture systems have enhanced the laboratory efficiency and the quality of the patient care. However, the lysis- centrifugation Isolator ® system, a conventional method, remains used for the diagnosis of infections due to fastidious bacteria and for the diagnosis of catheter-associated infections. Methods based on the 16S rDNA amplification, using universal primers, could be a convenient tool used for the management of blood culture negative endocarditis, even if their interest remains to be validated.
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