Abstract
Leptin, secreted by adipocytes, directly influences the onset of puberty in mammals. Our previous study showed that leptin stimulation could promote the secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from pituitary cells in primary culture and ovarian development in chub mackerel. This study aimed to elucidate the detailed mechanism of leptin-induced effects on gonadotropin hormone-producing cells. We produced recombinant leptin using silkworm pupae and investigated the effects of leptin on FSH and LH secretion and gene expression in the primary culture of pituitary cells from chub mackerel. The presence or absence of co-expression of lepr mRNA, FSH and LH b-subunit mRNA in gonadotropic cells was examined by double-labeled in situ hybridization. The addition of leptin significantly increased the secretion and gene expression of FSH and LH from male and female pituitary cells in primary culture. In contrast, gonadotropin-releasing hormone 1 affected neither FSH secretion in cells from females nor fshb and lhb expression in cells from males and females. The expression of lepr was observed in FSH- and LH-producing cells of both males and females. The results indicate that leptin directly regulates gonadotropin synthesis and secretion and plays an important role in the induction of puberty in teleost fish.
Highlights
Leptin is a 16 kDa hormone secreted by fat cells [1]
The open reading frame (ORF) of chub mackerel leptin-A was cloned into the modified pFastBac vector, pFastBac L21-GGcTEVdH8STREP, which contains the ccdB cassette flanked by two BsaI sites with unique overhangs for the Golden Gate cloning system [23]
In The our inefficiency previous studies, leptin waswere synthesized usingfaced an in the application of coli-produced leptin
Summary
Leptin is a 16 kDa hormone secreted by fat cells [1]. It mediates its signal through the membrane receptor LepR, which belongs to the class I cytokine receptor family [2].Adequate nutrition is required for the initiation of puberty, and leptin plays an important role in initial maturation by transmitting the amount of energy stored in the body to the reproductive axis [3]. Leptin is a 16 kDa hormone secreted by fat cells [1]. It mediates its signal through the membrane receptor LepR, which belongs to the class I cytokine receptor family [2]. Adequate nutrition is required for the initiation of puberty, and leptin plays an important role in initial maturation by transmitting the amount of energy stored in the body to the reproductive axis [3]. There is a high correlation between circulating leptin and gonadotropin (GTH) levels during the pubertal process [4]. Administration of leptin to the periphery can induce puberty efficiently [5,6]. Malnutrition reduces circulating leptin levels and suppresses sexual function; the condition can be rescued by leptin administration [7,8]
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