Abstract
BackgroundEast Java has become one of the provinces that have higher prevalence of leprosy, especially in the coastal region. Environment has also influenced for leprosy transmission and early detection could reduce the incidence rate of new leprosy cases. Epidemiological studies of leprosy in children can give an illustration of the important aspects of the environment. Presence of Mycobacterium leprae (M. leprae) DNA in nasal swabs and seropositivity level among them can describe M. leprae exposure in that area.ObjectiveAnalyzing PCR from nasal swab and seropositivity level among elementary school children between northern coast and southern coast of East Java province.MethodsFive hundred and thirty children in Pacitan and Lamongan were involved. Both areas are representation of northern and southern coastal region in East Java Province. After clinical examination, nasal swab and blood samples were obtained. ELISA test was performed to measure the titer of IgM anti Phenolic Glicolipid-1 (PGL-1) antibody then continued by PCR to detect M. leprae DNA.ResultsFrom 301 students in Pacitan, 25 students (8.3%) are sero-positives and 9 students (2.9%) are PCR positives. from 229 students in Lamongan, 110 (48,3%) students are sero-positives and 49 students (21.4%) are PCR positives. Both are analyzed by Chi-Square, and from the PCR and ELISA, it concluded that there are statistically significant differences between the two regions.ConclusionFrom study above shows that in the northern coast of East Java, incidence of subclinical leprosy is still high, it means that in this area still has a high risk of new cases of leprosy in the future and early detection tools need to performed as a preventative measure.
Highlights
Dinar Adriaty,[1] Cita Rosita SP,[1,2] Iswahyudi,[1] Ratna Wahyuni,[1] ease cause by Mycobacterium leprae (M. leprae). It is still become a major problem in Indonesia because it involves severe
Collection of clinical specimens Samples were taken by purposive sampling, from 4th to 6th grade elementary school children
Healthy school children lyzed by Chi-Square, and from the PCR and coast of east java province that representa- were taken from one village in the ELISA, it concluded that there are statisti- tive of leprosy and non leprosy endemic Lamongan district that represent the leprosy cally significant differences between the areas in East Java
Summary
Key words: Mycobacterium leprae, DNA, phenolic glycolipid-1, children, Indonesia. IndonesiaLeprosy is a chronic granulomatous dis- Contributions: The authors contributed equally.Dinar Adriaty,[1] Cita Rosita SP,[1,2] Iswahyudi,[1] Ratna Wahyuni,[1] ease cause by Mycobacterium leprae (M. leprae). It is still become a major problem in Indonesia because it involves severe
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