Lentivirus Vector Gene Therapy of Apoptin: The Expression of E-Catenin in Prostate Cancer Cells

Abstract

Lentivirus vectors are effective tools for gene therapy. The cloning of apoptosis-inducing genes in the malignant cell genome has been dramatically investigated in researches. In this study, the anti-cancer effect of the vector gene expressing the apoptin gene in the cellular class derived from prostate cancer was investigated. In this experimental study, an apoptotic expressive lenticular vector was developed to transfer the apoptin gene to the prostate cancer cell line. Then using the real time quantitative polymerase chain reaction fluorescence technique, transactional doctrine, and expression of the apoptin gene were evaluated, respectively. Subsequently, the anticancer effect of apoptin gene expression in the prostate cancer cell was investigated by evaluating the expression of E-cadherin. The rate of positive green fluorescent protein cells in prostate cancer cells increased by more than 80 % compared with control cells that had less than 1 % green fluorescent protein -positive cells expression of apoptin in cells infected with lentivirus vector- green fluorescent protein-Apoptin increased about 5-fold during 72 h (p=0.017). Lentivirus Apoptin treated cells had higher E-cadherin expression than the control group (p=0.013). The results of polymerase chain reaction showed that the lentivirus vector could affect prostate cancer cells by increasing the expression of E-cadherin gene expression in prostate cancer cells.