Abstract
Objective To investigate the expression of RFP in Schwann cell of rat sciatic nerve transfected with lentivirus vetor.Methods Sciatic nerve injury model was established by transection of right sciatic nerve in 60 female wistar rats that were then randomly divided into 4 groups:group A,B,C and D.At the end of the procedure,8μl of Lenti-RFP suspension (2 × 10-6 TU,2 × 10-7 TU,2 × 10-8 TU) were injected into the sciatic nerve of the rats in group B,C and D,respectively.The rats in group A received 8μl of balanced salt solution (BSS) and were used as the control.In the 1st,2nd and 4th week after the surgery,the expression of RFP and the effects of lentivirus vetor on Schwann cells were tested,and the transfection efficiency was calculated.Results RFP gene expressions were observed in Group B,C and D one week after surgery,and the highest transfection efficiency were found in group D at the 4th week after surgery..Statistically significant differences of transduction efficiency were observed in the different dosage groups at the same time,or in the same group at different time.The histological damage to sciatic nerve caused by the vector was not obvious in every group.Conclusions RFP gene can be transfected into Schwann cells in vivo with stable and efficient expression.Clear dosage-dependent and time-dependent features were observed.Lentivirus vector caused no histological damage to sciatic nerve. Key words: Lentiviral vector; Red fluorescent protein; Gene transduction
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