Abstract
Lentinan could exhibit significant biological activity favorable for human health and disease control such as the recovery of patients with liver cancer. In order to investigate the effect of lentinan dose dependence between immunoprophylaxis and promotion of cancer cell proliferation of the murine liver cancer, different concentrations of lentinan were prepared for the test in vitro (MTT assay) and in vivo (cumulative survival assay, spleen lymphocyte proliferation tests and peritoneal macrophage phagocytosis assays). New emerging proteins of the H22 cell incubated with lentinan was demonstrated by MS analysis and protein database searching. Lentinan was non-toxic for HL7702 cells but inhibited H22 cells proliferation obviously in a dose-dependent manner. In vivo, the proliferation of H22 hepatocarcinoma cells was inhibited by lentinan 0.4mg/kg body weight (L2, survival rate, 20%, PPP<0.01). Six proteins 60Sacidic ribosomal protein P2, Peroxiredoxin-2, Annexin A5, PDZ and LIM domain protein 1, Src substrate cortactin and Moesin were found as emerging proteins of the H22 cell incubated with high dose lentinan which related to cancer promotion closely. In conclusion, Thelentinan was relatively safe and could inhibit the proliferation of H22 cancer cells through immunity improvement when it's intake was in proper quantity.
Highlights
Polysaccharides belong to a class of natural polymers that are comprised of carbohydrate monomers linked by glycosidic linkages [1]
The proliferation of H22 hepatocarcinoma cells was inhibited by lentinan 0.4mg/kg body weight (L2, survival rate, 20%, P
Six proteins 60Sacidic ribosomal protein P2, Peroxiredoxin-2, Annexin A5, PDZ and LIM domain protein 1, Src substrate cortactin and Moesin were found as emerging proteins of the H22 cell incubated with high dose lentinan which related to cancer promotion closely
Summary
Polysaccharides belong to a class of natural polymers that are comprised of carbohydrate monomers linked by glycosidic linkages [1]. Polysaccharides possess various physicochemical properties such as gelation, solubility, low osmotic effect and surface properties which depend on their composition and chemical structure [2, 3]. A large number of studies have suggested that polysaccharides exhibit an anticancer effect following some common mechanisms: (1) prevention of oncogenesis by oral consumption of active preparations [8]; (2) enhancement of immunity against tumors directly or indirectly through combination with chemotherapy [9]; (3) inhibiting tumors such as induction of tumor cell apoptosis or inhibition of tumor metastasis [10, 11]; (4) activation of humoral and cellular immunity to prevent cancer cell proliferation [12]. Activity of immunity-enhancement is important function of lentinan, which has become a hot topic in lentinan study [13]. Regulation of the levels of lymphocytes’ proliferative activity and macrophages’ phagocytic activity, cytokines, and antibodies may contribute to lentinan’s immunityenhancement abilities [14, 15]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
