Lens Design for Small Diameter in Vivo Endoscopic Multiphoton Microscopy

Abstract

Multiphoton microscopy has demonstrated the capacity to image tissue anatomy and morphology with high resolution optical depth sectioning and the potential for diagnosis of tissue health. We are building devices to transition multiphoton imaging and diagnostic techniques from the laboratory to the clinic and hospital using in vivo multiphoton endoscopy. In this instrument, lens design for a miniature (< 5 mm outer diameter) endoscopic multiphoton microscopy system presents a significant challenge. Lens system design goals include: wide field of view and moderate resolution image acquisition, high epifluorescent collection efficiency from scattering media, design for use with scanned optical fiber imaging systems, small device size and low cost manufacture. We present results for a three-successive element lens design that meets these criteria.